Global metabolite analysis of yeast: evaluation of sample preparation methods.

  title={Global metabolite analysis of yeast: evaluation of sample preparation methods.},
  author={Silas Granato Villas-B{\^o}as and Jesper H\ojer-Pedersen and Mats Akesson and J\orn Smedsgaard and Jens Nielsen},
  volume={22 14},
Sample preparation is considered one of the limiting steps in microbial metabolome analysis. Eukaryotes and prokaryotes behave very differently during the several steps of classical sample preparation methods for analysis of metabolites. Even within the eukaryote kingdom there is a vast diversity of cell structures that make it imprudent to blindly adopt protocols that were designed for a specific group of microorganisms. We have therefore reviewed and evaluated the whole sample preparation… CONTINUE READING
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