Global metabolic profiling procedures for urine using UPLC–MS

  title={Global metabolic profiling procedures for urine using UPLC–MS},
  author={Elizabeth J. Want and Ian D Wilson and Helen G Gika and Georgios Theodoridis and Robert S. Plumb and John P. Shockcor and Elaine Holmes and Jeremy K. Nicholson},
  journal={Nature Protocols},
The production of 'global' metabolite profiles involves measuring low molecular-weight metabolites (<1 kDa) in complex biofluids/tissues to study perturbations in response to physiological challenges, toxic insults or disease processes. Information-rich analytical platforms, such as mass spectrometry (MS), are needed. Here we describe the application of ultra-performance liquid chromatography–MS (UPLC–MS) to urinary metabolite profiling, including sample preparation, stability/storage and the… CONTINUE READING
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