Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution

@article{Lee2012GlobalMO,
  title={Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution},
  author={Sooncheol Lee and Botao Liu and Soohyun Lee and Sheng-Xiong Huang and Ben Shen and Shu-Bing Qian},
  journal={Proceedings of the National Academy of Sciences},
  year={2012},
  volume={109},
  pages={E2424 - E2432}
}
Understanding translational control in gene expression relies on precise and comprehensive determination of translation initiation sites (TIS) across the entire transcriptome. The recently developed ribosome-profiling technique enables global translation analysis, providing a wealth of information about both the position and the density of ribosomes on mRNAs. Here we present an approach, global translation initiation sequencing, applying in parallel the ribosome E-site translation inhibitors… Expand
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TLDR
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TLDR
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TLDR
A new method, TISCA (TIS detection by translation Complex Analysis), is described for the accurate identification of TISs, found to be more reliable for TIS detection compared with existing tools, and it identified a substantial number of near-cognate codons in Kozak-like sequence contexts. Expand
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