Global kinetic analysis of seeded BSA aggregation.

@article{Sahin2016GlobalKA,
  title={Global kinetic analysis of seeded BSA aggregation.},
  author={Ziya Sahin and Yusuf Kemal Demir and Veysel Kayser},
  journal={European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences},
  year={2016},
  volume={86},
  pages={115-24}
}
Accelerated aggregation studies were conducted around the melting temperature (Tm) to elucidate the kinetics of seeded BSA aggregation. Aggregation was tracked by SEC-HPLC and intrinsic fluorescence spectroscopy. Time evolution of monomer, dimer and soluble aggregate concentrations were globally analysed to reliably deduce mechanistic details pertinent to the process. Results showed that BSA aggregated irreversibly through both sequential monomer addition and aggregate-aggregate interactions… CONTINUE READING
1 Citations
53 References
Similar Papers

Citations

Publications citing this paper.

References

Publications referenced by this paper.
Showing 1-10 of 53 references

Kinetic analysis of the multistep aggregation mechanism of monoclonal antibodies

  • L Nicoud
  • Journal of Physical Chemistry B,
  • 2014
Highly Influential
8 Excerpts

Similar Papers

Loading similar papers…