Global gene silencing is caused by the dissociation of RNA polymerase II from DNA in mouse oocytes.

@article{Abe2010GlobalGS,
  title={Global gene silencing is caused by the dissociation of RNA polymerase II from DNA in mouse oocytes.},
  author={Ken-Ichiro Abe and Azusa Inoue and Masataka G. Suzuki and Fugaku Aoki},
  journal={The Journal of reproduction and development},
  year={2010},
  volume={56 5},
  pages={502-7}
}
As mouse oocytes approach maturity, a global repression of gene transcription occurs. Here, we investigated the involvement of RPB1, the largest subunit of RNA polymerase II (RNAP II), in the regulation of this transcriptional silencing mechanism. Using BrUTP to follow transcription in an in vitro run-on assay, we observed an abrupt decrease in transcriptional activity when oocytes reached their full size (approximately 80 µm). Immunoblotting using antibodies specific for the phosphorylated and… CONTINUE READING

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