Global and quantitative profiling of polyadenylated RNAs using PAS-seq.

Abstract

mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of… (More)
DOI: 10.1007/978-1-62703-971-0_16

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Cite this paper

@article{Yao2014GlobalAQ, title={Global and quantitative profiling of polyadenylated RNAs using PAS-seq.}, author={Chengguo Yao and Yongsheng Shi}, journal={Methods in molecular biology}, year={2014}, volume={1125}, pages={179-85} }