Global and local regulation of clathrin-coated pit dynamics detected on patterned substrates.

@article{Liu2009GlobalAL,
  title={Global and local regulation of clathrin-coated pit dynamics detected on patterned substrates.},
  author={Allen P Liu and Dinah Loerke and Sandra L Schmid and Gaudenz Danuser},
  journal={Biophysical journal},
  year={2009},
  volume={97 4},
  pages={1038-47}
}
Live-cell imaging of individual clathrin-coated pit (CCP) dynamics has revealed a broad variation in their internalization kinetics, but the functional significance and mechanistic underpinnings of this heterogeneity remain unknown. One contributing factor may be the spatial variations in the underlying actin cortex. To test this, we cultured cells on fibronectin (Fn) micropatterned substrates to vary the cortical actin mechanics in a defined manner. Under these conditions, stress fibers became… CONTINUE READING