Global analysis of metabolites in rat and human urine based on gas chromatography/time-of-flight mass spectrometry.

@article{Jiye2008GlobalAO,
  title={Global analysis of metabolites in rat and human urine based on gas chromatography/time-of-flight mass spectrometry.},
  author={A Jiye and Qing Huang and Guang-ji Wang and Weibin Zha and Bei Yan and Hongcan Ren and Shenghua Gu and Ying Zhang and Qi Zhang and Feng Shao and Longsheng Sheng and Jingjng Sun},
  journal={Analytical biochemistry},
  year={2008},
  volume={379 1},
  pages={20-6}
}
Sediment in urine may contain low-molecular-weight compounds that should be included in the analysis. To date, no systematic investigation has addressed this issue. We investigated three primary factors that influence the extraction efficiency of metabolites during preparation of urine samples for metabolomic research: centrifugation, pH, and extraction solvents. Obtained with the use of gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) technique and principal component analysis… CONTINUE READING