Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains.

@article{Liu2014GlobalAO,
  title={Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains.},
  author={Bo Liu and Gintaras Deikus and Anna C Bree and Sylvain Durand and Daniel B Kearns and David H. Bechhofer},
  journal={Molecular microbiology},
  year={2014},
  volume={94 1},
  pages={41-55}
}
Messenger RNA decay in Bacillus subtilis is accomplished by a combination of exoribonucleases and endoribonucleases. Intermediates in the decay process have not been readily detectable, and previous studies on mRNA decay have used a handful of highly expressed transcripts as models. Here, we use RNA-Seq analysis to probe mRNA turnover globally. A significant fraction of messages showed differential accumulation of RNA fragments that mapped near the 5' or 3' end of the coding sequence… CONTINUE READING
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