Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.

@article{Holt2009GlobalAO,
  title={Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.},
  author={Liam J Holt and Brian B. Tuch and Judit Vill{\'e}n and Alexander Johnson and Steven P Gygi and David O. Morgan},
  journal={Science},
  year={2009},
  volume={325 5948},
  pages={1682-6}
}
To explore the mechanisms and evolution of cell-cycle control, we analyzed the position and conservation of large numbers of phosphorylation sites for the cyclin-dependent kinase Cdk1 in the budding yeast Saccharomyces cerevisiae. We combined specific chemical inhibition of Cdk1 with quantitative mass spectrometry to identify the positions of 547… CONTINUE READING