Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy.

@article{Brownridge2011GlobalAQ,
  title={Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy.},
  author={Philip J. Brownridge and Stephen W. Holman and Simon J. Gaskell and Chris M Grant and Victoria M. Harman and Simon J. Hubbard and Karin Lanthaler and Craig Lawless and Ronan O'Cualain and Paul Francis George Sims and Rachel M. Watkins and Robert J Beynon},
  journal={Proteomics},
  year={2011},
  volume={11 15},
  pages={2957-70}
}
In this paper, we discuss the challenge of large-scale quantification of a proteome, referring to our programme that aims to define the absolute quantity, in copies per cell, of at least 4000 proteins in the yeast Saccharomyces cerevisiae. We have based our strategy on the well-established method of stable isotope dilution, generating isotopically labelled peptides using QconCAT technology, in which artificial genes, encoding concatenations of tryptic fragments as surrogate quantification… CONTINUE READING

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