Global Survey of Genomic Imprinting by Transcriptome Sequencing

@article{Babak2008GlobalSO,
  title={Global Survey of Genomic Imprinting by Transcriptome Sequencing},
  author={Tomas Babak and Brian DeVeale and Christopher D. Armour and Christopher Raymond and Michele A. Cleary and Derek van der Kooy and Jason M. Johnson and Lee P. Lim},
  journal={Current Biology},
  year={2008},
  volume={18},
  pages={1735-1741}
}
Genomic imprinting restricts gene expression to a paternal or maternal allele. To date, approximately 90 imprinted transcripts have been identified in mouse, of which the majority were detected after intense interrogation of clusters of imprinted genes identified by phenotype-driven assays in mice with uniparental disomies [1]. Here we use selective priming and parallel sequencing to measure allelic bias in whole transcriptomes. By distinguishing parent-of-origin bias from strain-specific bias… 
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This article focuses on three common sources of potential confounding effects: (i) random monoallelic expression in monoclonal cell populations, (ii) genetically determined monoalle Alic expression and (iii) contamination or infiltration of embryonic tissues with maternal material.
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