Global Quantitative SILAC Phosphoproteomics Reveals Differential Phosphorylation Is Widespread between the Procyclic and Bloodstream Form Lifecycle Stages of Trypanosoma brucei

@inproceedings{Urbaniak2013GlobalQS,
  title={Global Quantitative SILAC Phosphoproteomics Reveals
Differential Phosphorylation Is Widespread between the Procyclic and
Bloodstream Form Lifecycle Stages of Trypanosoma brucei},
  author={Michael D. Urbaniak and David M. A. Martin and Michael A. J. Ferguson},
  booktitle={Journal of proteome research},
  year={2013}
}
We report a global quantitative phosphoproteomic study of bloodstream and procyclic form Trypanosoma brucei using SILAC labeling of each lifecycle stage. Phosphopeptide enrichment by SCX and TiO2 led to the identification of a total of 10096 phosphorylation sites on 2551 protein groups and quantified the ratios of 8275 phosphorylation sites between the two lifecycle stages. More than 9300 of these sites (92%) have not previously been reported. Model-based gene enrichment analysis identified… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 71 CITATIONS, ESTIMATED 58% COVERAGE

Flagellum inheritance in Trypanosoma brucei requires a kinetoplastid-specific protein phosphatase.

  • The Journal of biological chemistry
  • 2018
VIEW 6 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2013
2019

CITATION STATISTICS

  • 5 Highly Influenced Citations

  • Averaged 12 Citations per year from 2017 through 2019

References

Publications referenced by this paper.
SHOWING 1-10 OF 39 REFERENCES

Comparative proteomics of two life cycle stages of stable isotope - labelled Trypanosoma brucei reveals novel components of the parasite ’ s host adaption machinery

K. Fenn, K. R. Matthews
  • Mol . Cell . Proteomics
  • 2013

Expression of the RNA recognition motif protein RBP 10 promotes a bloodstream - form transcript pattern in Trypanosoma brucei

S. Dean, R. Marchetti, K. Kirk, K. R. Matthews
  • Mol . Microbiol .
  • 2012