Global, In Vivo, and Site-Specific Phosphorylation Dynamics in Signaling Networks

  title={Global, In Vivo, and Site-Specific Phosphorylation Dynamics in Signaling Networks},
  author={J. Olsen and B. Blagoev and F. Gnad and B. Ma{\vc}ek and C. Kumar and P. Mortensen and M. Mann},
  • J. Olsen, B. Blagoev, +4 authors M. Mann
  • Published 2006
  • Biology, Medicine
  • Cell
  • Cell signaling mechanisms often transmit information via posttranslational protein modifications, most importantly reversible protein phosphorylation. Here we develop and apply a general mass spectrometric technology for identification and quantitation of phosphorylation sites as a function of stimulus, time, and subcellular location. We have detected 6,600 phosphorylation sites on 2,244 proteins and have determined their temporal dynamics after stimulating HeLa cells with epidermal growth… CONTINUE READING
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