GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli.

@article{Araujo2004GlnBIS,
  title={GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli.},
  author={Luiza Maria Araujo and Rose Adele Monteiro and Emanuel Maltempi de Souza and Maria Berenice Reynaud Steffens and Liu Un Rigo and F{\'a}bio de Oliveira Pedrosa and Leda Satie Chubatsu},
  journal={Research in microbiology},
  year={2004},
  volume={155 6},
  pages={491-5}
}
The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were expressed in Escherichia coli and analyzed for their ability to activate nif gene expression. When expressed separately, none of the proteins were able to activate nifH promoter expression in any tested conditions; in contrast, nifH expression was observed in cells grown in the absence of ammonium and oxygen and when expressing simultaneously NifA and GlnB proteins, but… CONTINUE READING

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