Glioblastoma-derived spheroid cultures as an experimental model for analysis of EGFR anomalies

@inproceedings{WitusikPerkowska2010GlioblastomaderivedSC,
  title={Glioblastoma-derived spheroid cultures as an experimental model for analysis of EGFR anomalies},
  author={Monika Witusik-Perkowska and Piotr Rieske and Krystyna Hułas-Bigoszewska and Magdalena Zakrzewska and Robert Stawski and Dominika Kulczycka-Wojdala and Michał Bieńkowski and Ewelina Stoczyńska-Fidelus and Sylwia M. Gre{\vs}ner and Sylwester Piaskowski and Dariusz Jan Jask{\'o}lski and Wielisław Papierz and Krzysztof Marcin Zakrzewski and Maciej Kolasa and James W Ironside and Paweł P Liberski},
  booktitle={Journal of Neuro-Oncology},
  year={2010}
}
Glioblastoma cell cultures in vitro are frequently used for investigations on the biology of tumors or new therapeutic approaches. Recent reports have emphasized the importance of cell culture type for maintenance of tumor original features. Nevertheless, the ability of GBM cells to preserve EGFR overdosage in vitro remains controversial. Our experimental approach was based on quantitative analysis of EGFR gene dosage in vitro both at DNA and mRNA level. Real-time PCR data were verified with a… CONTINUE READING
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