Glioblastoma cell line-derived spheres in serum‑containing medium versus serum-free medium: a comparison of cancer stem cell properties.

@article{Hong2012GlioblastomaCL,
  title={Glioblastoma cell line-derived spheres in serum‑containing medium versus serum-free medium: a comparison of cancer stem cell properties.},
  author={Xin Hong and Khalil J Chedid and Steven N. Kalkanis},
  journal={International journal of oncology},
  year={2012},
  volume={41 5},
  pages={1693-700}
}
In addition to the primary culturing of cancer stem cells (CSCs) from tumor tissues, CSCs are found in established tumor cell lines. However, it is unclear how culture conditions affect CSC enrichment. Additionally, the differentiation potential of cell line-derived CSCs has not been well studied. In our study, the glioblastoma cell lines LN229, T98G, U251n and U87, were cultured as spheres in serum-containing medium (serum spheres) or… CONTINUE READING
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