Glimpses of a Tiny RNA World

@article{Ruvkun2001GlimpsesOA,
  title={Glimpses of a Tiny RNA World},
  author={Gary Ruvkun},
  journal={Science},
  year={2001},
  volume={294},
  pages={797 - 799}
}
  • G. Ruvkun
  • Published 26 October 2001
  • Biology
  • Science
Naturally tiny regulatory RNAs of about 22 nucleotides were first identified from Caenorhabditis elegans genetics. Artificially induced RNAs of this size are also an intermediate in RNA interference, and both such RNAs use a common biochemical processing pathway. Now three groups have shown that there is an entire world of tiny RNAs that had almost escaped detection until now. These tiny RNAs are likely to regulate the translation of other mRNAs during development and, like the intermediates… 
An Expanding Universe of Noncoding RNAs
Noncoding RNAs (ncRNAs) have been found to have roles in a great variety of processes, including transcriptional regulation, chromosome replication, RNA processing and modification, messenger RNA
Short silencing RNA: the dark matter of genetics?
  • D. Baulcombe
  • Biology, Medicine
    Cold Spring Harbor symposia on quantitative biology
  • 2006
TLDR
The possibility that this RDR-generated dsRNA initiates a cascade in which there are multiple rounds of secondary sRNA production is discussed, which is proposed to feature in mechanisms that can either buffer mRNA populations against change or mediate extensive changes in mRNA populations.
A biochemical framework for RNA silencing in plants.
TLDR
This finding supports the view that plant miRNAs direct RNAi and that miRNA-specified mRNA destruction is important for proper plant development and endonuclease complexes guided by small RNAs are a common feature of RNA silencing in both animals and plants.
Why do miRNAs live in the miRNP?
TLDR
Identifying the ribonucleoprotein complex (RNP) associated with a noncoding RNA gives clues to its cellular function and biochemical mechanism by revealing the proteins whose company it keeps.
The genetics of RNA silencing.
TLDR
This chapter gives an overview of both biochemical and genetic approaches leading to the current understanding of the molecular mechanism of RNA silencing and its probable biological function.
Biochemical Mechanism of RNA Interference in Higher Organisms: A Dissertation
TLDR
Designing siRNAs that target the single nucleotide polymorphism in superoxide dismutase that causes the familial form of amyotrophic lateral sclerosis (ALS), but leave the wild-type mRNA intact and functional is used.
Computational Prediction on Mammalian and Viral MicroRNAs - a Review
TLDR
In 1993, a small non-coding RNA, lin-4, was identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3′ untranslated region (UTR) of lin-14 mRNA, and was termed as small temporal RNA or stRNA due to its temporal expression during the first larval stage.
Noncoding RNAs: A Regulatory Role?
Around 97–98% of the transcriptional output of the human genome is noncoding RNA, which is derived from noncoding RNA genes and from introns in protein-coding genes. These RNAs may form a
Computational identification and analysis of noncoding RNAs - Unearthing the buried treasures in the genorne
TLDR
The central dogma of molecular biology states that the genetic information flows from DNA to RNA to protein, and RNA was mainly viewed as a passive intermediary that bridges the gap between DNA and protein.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 19 REFERENCES
An Extensive Class of Small RNAs in Caenorhabditis elegans
  • Rosalind C. Lee, V. Ambros
  • Biology, Medicine
    Science
  • 2001
TLDR
Using bioinformatics and cDNA cloning, this work found 15 new miRNA genes in C. elegans that express small transcripts that vary in abundance during larval development, and three of them have apparent homologs in mammals and/or insects.
An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans
TLDR
Two small temporal RNAs, lin-4 andlet-7, control developmental timing in Caenorhabditis elegans and are found to be members of a large class of 21- to 24-nucleotide noncodingRNAs, called microRNAs (miRNAs), which imply that, as a class, miRNAs have broad regulatory functions in animals.
A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA
TLDR
In Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA, which regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals.
Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi
TLDR
Biochemical purification of the RNAi effector nuclease from cultured Drosophila cells reveals that one constituent of this complex is a member of the Argonaute family of proteins, which are essential for gene silencing in Caenorhabditis elegans, Neurospora, and Arabidopsis.
Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
TLDR
Two small RNAs regulate the timing of Caenorhabditis elegans development and may control late temporal transitions during development across animal phylogeny.
A species of small antisense RNA in posttranscriptional gene silencing in plants.
TLDR
The 25-nucleotide antisense RNA detected in transgene-induced PTGS is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.
RNA interference is mediated by 21- and 22-nucleotide RNAs.
TLDR
It is demonstrated that 21- and 22-nt RNA fragments are the sequence-specific mediators of RNAi, and evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the siRNA-protein complex is provided.
...
1
2
...