Glial changesin vitro induced by the inhibitor of succinic dehydrogenase


The object of the experiment was to obtainin vitro the glial changes typical of hepatogenic encephalopathy, after inhibition of one of the stages of the Krebs cycle. A chemical inhibitor of succinic dehydrogenase, sodium malonate, was added to the culture medium in various concentrations. The experiment was carried out in four groups. The damage to the metabolism of the Krebs cycle resulted in the appearance of typical Opalski cells and intermediate cell forms. These pathological cells exhibited the same morphological and histochemical properties as those observed in experimental hepatogenic encephalopathy in vivo and in vitro. The striking feature of the Opalski cells was a significant decrease of SDH and GDH activity. The authors discuss the possible pathogenesis of the glial changes which are probably due to the disturbance of ammonia detoxication.

DOI: 10.1007/BF00697746

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@article{Renkawek1973GlialCV, title={Glial changesin vitro induced by the inhibitor of succinic dehydrogenase}, author={Krystyna Renkawek and Zuzanna Kr{\'a}snicka and T. Majdecki and Miroslaw J. Mossakowski}, journal={Acta Neuropathologica}, year={1973}, volume={26}, pages={107-114} }