Glass surfaces grafted with high-density poly(ethylene glycol) as substrates for DNA oligonucleotide microarrays.

@article{Schlapak2006GlassSG,
  title={Glass surfaces grafted with high-density poly(ethylene glycol) as substrates for DNA oligonucleotide microarrays.},
  author={Robert Schlapak and Patrick Pammer and David Armitage and Ruiyue Zhu and P. Hinterdorfer and Matthias Vaupel and Thomas Fr{\"u}hwirth and Stefan Howorka},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2006},
  volume={22 1},
  pages={277-85}
}
Surfaces carrying a dense layer of poly(ethylene glycol) (PEG) were prepared, characterized, and tested as substrates for DNA oligonucleotide microarrays. PEG bis(amine) with a molecular weight of 2000 was grafted onto silanized glass slides bearing aldehyde groups. After grafting, the terminal amino groups of the PEG layer were derivatized with the heterobifunctional cross-linker succinimidyl 4-[p-maleimidophenyl]butyrate to permit the immobilization of thiol-modified DNA oligonucleotides. The… CONTINUE READING

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