Glass slide smears are a suitable source for RT-PCR-based analysis of chromosomal aberrations in leukaemias.

Abstract

The polymerase chain reaction (PCR) is a well-established method to detect cytogenetic abnormalities. The handling of fresh specimens is difficult, therefore a method to use smears of blood or bone marrow as a source would be advantageous. Furthermore, such a technique would give the opportunity to investigate retrospectively bone marrow smears in… (More)

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