Ginger rhizome as a potential source of milk coagulating cysteine protease.

@article{Hashim2011GingerRA,
  title={Ginger rhizome as a potential source of milk coagulating cysteine protease.},
  author={Malik Muhammad Hashim and Dong Mingsheng and Muhammad Farooq Iqbal and Chen Xiaohong},
  journal={Phytochemistry},
  year={2011},
  volume={72 6},
  pages={458-64}
}
A milk coagulating protease was purified ∼10.2-fold to apparent homogeneity from ginger rhizomes in 34.9% recovery using ammonium sulfate fractionation, together with ion exchange and size exclusion chromatographic techniques. The molecular mass of the purified protease was estimated to be ∼36kDa by SDS-PAGE, and exhibited a pI of 4.3. It is a glycoprotein with 3% carbohydrate content. The purified enzyme showed maximum activity at pH 5.5 and at a temperature of ∼60°C. Its protease activity was… CONTINUE READING
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Showing 1-10 of 53 references

Characterization of ginger proteases and their potential as a rennin replacement

  • H. P. Su, M. J. Huang, H. T. Wang
  • J. Sci. Food Agric
  • 2009
Highly Influential
7 Excerpts

Proteolysis during cheese manufacture and ripening

  • P. F. Fox
  • J. Dairy Sci
  • 1989
Highly Influential
4 Excerpts

Proteolysis of whey proteins

  • S. Castro, D. V. Peyronel, A. B. Cantera
  • 1996
Highly Influential
3 Excerpts

Protease assay methods

  • G. Sarath, R. S. Motte, F. M. Wanger
  • 1989
Highly Influential
3 Excerpts

Proteolysis during the ripening of goats’ milk cheese made with plant coagulant or calf rennet

  • A. Pino, F. Prados, E. Galán, P.L.H. McSweeney, J. Fernández-Salguero
  • Food Res. Int
  • 2009
1 Excerpt

Proteolytic activity in enzymatic extracts from Carica papaya L . cv . Maradol harvest by - products

  • G. E. Thomás, H. G. Rodolfo, +4 authors G. T. Santiago
  • Process Biochem .
  • 2009
1 Excerpt

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