Gibberellin perception at the plasma membrane of Avena fatua aleurone protoplasts

@article{Hooley1991GibberellinPA,
  title={Gibberellin perception at the plasma membrane of Avena fatua aleurone protoplasts},
  author={Richard A. Hooley and Michael H. Beale and Sally J. Smith},
  journal={Planta},
  year={1991},
  volume={183},
  pages={274-280}
}
A functional assay for gibberellin (GA) receptors is described based on the induction of α-amylase gene expression in isolated aleurone protoplasts of Avena fatua L. by GA4 immobilised to Sepharose beads. A 17-thiol derivative of GA4, shown to be biologically active with aleurone protoplasts, has been coupled to epoxy-activated Sepharose 6B. This GA4-17-Sepharose induces high levels of α-amylase when incubated with isolated aleurone protoplasts, while cells of the intact aleurone layer do not… CONTINUE READING
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