Gibberellin biosynthesis in Gibberella fujikuroi: cloning and characterization of the copalyl diphosphate synthase gene

@article{Tudzynski1998GibberellinBI,
  title={Gibberellin biosynthesis in Gibberella fujikuroi: cloning and characterization of the copalyl diphosphate synthase gene},
  author={Bettina Tudzynski and Hiroshi Kawaide and Yuji Kamiya},
  journal={Current Genetics},
  year={1998},
  volume={34},
  pages={234-240}
}
Abstract The gene coding for copalyl diphosphate synthase (CPS), which represents the first gene of the gibberellin pathway, was isolated from the rice pathogen Gibberella fujikuroi. This fungus is used commercially for the production of gibberellic acid and related gibberellins. CPS is a terpene cyclase which catalyzes the first specific step of the gibberellin (GA) pathway as it branches off from the general isoprenoid (biosynthetic) pathway at geranylgeranyl disphosphate (GGDP). A cDNA… 

Gibberellin biosynthetic pathway in Gibberella fujikuroi: evidence for a gene cluster.

Results suggest that at least some of the genes involved in the biosynthesis of gibberellins are closely linked in a gene cluster in G. fujikuroi, as has been recently found for other "dispensable" pathways in fungi.

Isolation and Characterization of the Gibberellin Biosynthetic Gene Cluster in Sphaceloma manihoticola

The close similarity between the two GA gene clusters, the identical gene functions, and the conserved intron positions suggest a common evolutionary origin despite the distant relatedness of the two fungi.

Biosynthesis of gibberellins in Gibberella fujikuroi: biomolecular aspects

  • B. Tudzynski
  • Biology
    Applied Microbiology and Biotechnology
  • 1999
This review gives a short overview of the well-known physiological and biochemical studies and concentrates mainly on the new molecular genetic data from GA research, including new information on the regulation of GA biosynthesis.

The P450-4 Gene of Gibberella fujikuroi Encodes ent-Kaurene Oxidase in the Gibberellin Biosynthesis Pathway

Gas chromatography-mass spectrometry analysis of extracts of mycelia and culture fluid of a P450-4 knockout mutant identified ent-kaurene as the only intermediate of the gibberellin biosynthesis pathway, and data suggest that P 450-4 encodes a multifunctional ent- Kaurene oxidase catalyzing all three oxidation steps betweenent-KAurene and ent-kasurenoic acid.

Characterization of the Final Two Genes of the Gibberellin Biosynthesis Gene Cluster of Gibberella fujikuroi

It is demonstrated by gene disruption that P450-3, one of the four cytochrome P450 monooxygenase genes in the GA gene cluster, encodes the 13-hydroxylase, which catalyzes the conversion of GA7 to GA3, in the last step of the pathway.

Isolation, characterization and disruption of the areA nitrogen regulatory gene of Gibberella fujikuroi

To generate areA-GF − mutants, gene replacement experiments were performed using a linearized replacement vector carrying the hygromycin B phosphotransferase (hph) gene, which resulted in transformants which were unable to utilize nitrogen sources other than ammonium and glutamine, and gave significantly reduced gibberellin production yields.

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Results suggest regulatory connections between carotenoid biosynthesis and nitrogen-controlled biosynthetic pathways in this fungus.

Conserved bases for the initial cyclase in gibberellin biosynthesis: from bacteria to plants.

Functional conservation of CPSs from bacteria to plants are suggested, presumably reflecting an early role for derived diterpenoids in both plant development and plant-microbe interactions, eventually leading to GA, and a speculative evolutionary scenario is presented.

Biochemical and Molecular Analyses of Gibberellin Biosynthesis in Fungi

  • H. Kawaide
  • Biology, Chemistry
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  • 2006
A molecular analysis of GA biosynthesis in Phaeosphaeria sp.
...

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