Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co2+

  title={Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co2+},
  author={Seung-Hyun Park and Masatoshi Nakajima and Masayuki Sakane and Zhengjun Xu and Katsushi Tomioka and Isomaro Yamaguchi},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={1498 - 1507}
Five full-length cDNA encoding gibberellin 2-oxidases, VaGA2oxA1, VaGA2oxA2, VaGA2oxB1, VaGA2oxB2, and VaGA2oxB3, were cloned from etiolated adzuki bean (Vigna angularis cv. Dainagon) seedlings, and their enzymatic characteristics were examined using recombinant enzymes fused with glutathione S-transferase (GST). Recombinant VaGA2oxA1 (rVaGA2oxA1) and rVaGA2oxA2 showed 2β-hydroxylation activity by converting GA1, GA4, GA9, GA20, GA4-methyl ester, and 16,17-dihydro-GA4 to the corresponding 2… 

Isolation and expression of GA 2-oxidase2 in tomato

  • J. XiaoJ. H. Zhang Z. Ye
  • Biology, Medicine
    DNA sequence : the journal of DNA sequencing and mapping
  • 2007
Semi-quantitative RT-PCR analysis showed a specific expression profile of SlGA2ox2 in different tissues, which mainly expressed in flowers and traces were detected in roots, stems, leaves and immature fruits.

Gibberellin receptor and its role in gibberellin signaling in plants.

It has been concluded that GID1 is a soluble receptor mediating GA signaling in rice, which binds to SLR1, a repressor of GA signaling, in a GA-dependent manner in yeast cells.

Trails to the gibberellin receptor, GIBBERELLIN INSENSITIVE DWARF1

The history of the research on GA receptor from the early attempts to the identification of GID1 is described, suggesting that the active principle of the gibberellin-binding protein was a G ID1 homolog.

Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt

The findings suggest that EVA inhibits angiogenesis in VEGF-treated HUVECs via inhibition of phosphorylation of VEGFR2, ERK, and Akt, and overall, the antiangiogenic mechanism of EVA is suggested to be inhibited.

Antheridiogen determines sex in ferns via a spatiotemporally split gibberellin synthesis pathway

It is determined that ferns have evolved an antheridiogen-mediated communication system to produce males by modifying the gibberellin biosynthetic pathway, which is split between two individuals of different developmental stages in the colony.

Identification of a Peptide Mimic of Bioactive Gibberellins with Affinity to GA 2-Oxidase

The first example of peptide mimics of a small hydrophobic molecule, a phytohormone gibberellin, has been reported and the second peptide mimic has been identified from random peptide libraries by its affinity to a type of catalyzing enzyme of gibBerellins, which specifically recognizes bioactive gibb Berellins.

Interacción de Giberelinas y Auxinas en la fructificación del tomate.

El efecto de la aplicacion de giberelinas (GAs) y auxinas durante la fructificacion y desarrollo del fruto, ha sido investigado en tomate (Solanum lycopersicum L.) cv Micro-Tom. Los resultados

Distribution and prediction of catalytic domains in 2-oxoglutarate dependent dioxygenases

  • S. Kundu
  • Biology, Computer Science
    BMC Research Notes
  • 2012
The proposed stratification is a novel attempt at classifying and predicting 2-oxoglutarate dependent function and will aid efforts by investigators to screen and characterize putative 2-OG dependent sequences by helping to compare data to a comprehensive list of HMM profiles of catalytic domains.

Similarities and Differences between the Characteristics of Gibberellin-Binding Protein and Gibberellin 2-Oxidases in Adzuki Bean (Vigna angularis) Seedlings

A major component that showed GA-binding activity was apparently different from any gibberellin 2-oxidase cloned from the seedlings, and it was not affected by the antibody treatment.



Gibberellin 2-oxidation and the SLN gene of Pisum sativum.

The expression patterns of the genes, together with the effects of the sln mutation, indicate that PsGA2ox1 plays a major role in GA20 deactivation in both shoots and maturing seeds, while the PsGA 2ox2 gene might be important for GA1 de activation in the shoot.

Molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation.

Functional screening of a cDNA library from developing cotyledons of runner bean with a highly sensitive tritium-release assay for enzyme activity revealed an up-regulation of GA 2-oxidase gene expression by GA, which contrasts GA-induced down- regulation of genes encoding the biosynthetic enzymes GA 20-Oxidase and GA 3beta-hydroxylase.

Expression Studies of Gibberellin Oxidases in Developing Pumpkin Seeds1

Results indicate tissue-specific GA-biosynthetic pathways operating within the developing seed as well as mRNA molecules of the 3-ox and 2-ox genes found in the embryo only.

Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice.

Results indicate that OsGA2ox1 encodes a GA 2-oxidase, which is functional not only in vitro but also in vivo, and suggests that reduction in its expression may contribute to the early development of the inflorescence meristem.

Gibberellin Induces α-Amylase Gene in Seed Coat of Ipomoea nil Immature Seeds

The RNA-blot analysis for these clones revealed that the InGA3ox2 gene was organ-specifically expressed in the developing seeds at 6–18 days after anthesis, supporting the idea that GAs play an important role in seed development by inducing α-amylase.

Deactivation of Gibberellin by 2-Oxidation during Germination of Photoblastic Lettuce Seeds

Gibberellin (GA) plays an important role in the induction of germination of photoblastic lettuce (Lactuca sativa L. cv. Grand Rapids) seeds. We have previously shown that gene expression of a GA

Differential Regulation of RNA Levels of Gibberellin Dioxygenases by Photoperiod in Spinach1

It is concluded that regulation and maintenance of GA1 concentration in spinach are primarily attributable to changes in expression of SoGA20ox1.

The SLENDER gene of pea encodes a gibberellin 2-oxidase.

RFLP analysis of a seedling population segregating for SLN and sln alleles showed the homozygous mutant allele co-segregating with the characteristic slender phenotype, concluding that SLN encodes GA 2-oxidase.

Internode length in Lathyrus odoratus. Effects of mutants l and lb on gibberellin metabolism and levels

Results strongly indicate that in genotype ll internode length is reduced and leaf growth altered by a reduction in GA1 levels attributable to a partial block in the 3β-hydroxylation of GA20 to GA1, consistent with the suggestion that lb alters some aspect of GA sensitivity.

GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their Regulation.

  • P. HeddenY. Kamiya
  • Biology
    Annual review of plant physiology and plant molecular biology
  • 1997
Highlights include the cloning of ent-copalyl diphosphate synthase and ent-kaurene synthase, and the demonstration that the former is targeted to the plastid; the finding that the Dwarf-3 gene of maize encodes a cytochrome P450, although of unknown function; and the clones of GA 20-oxidase and 3beta-hydroxylase genes.