Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co2+

@article{Park2005Gibberellin2F,
  title={Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co2+},
  author={Seung-Hyun Park and Masatoshi Nakajima and Masayuki Sakane and Zhengjun Xu and Katsushi Tomioka and Isomaro Yamaguchi},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2005},
  volume={69},
  pages={1498 - 1507}
}
Five full-length cDNA encoding gibberellin 2-oxidases, VaGA2oxA1, VaGA2oxA2, VaGA2oxB1, VaGA2oxB2, and VaGA2oxB3, were cloned from etiolated adzuki bean (Vigna angularis cv. Dainagon) seedlings, and their enzymatic characteristics were examined using recombinant enzymes fused with glutathione S-transferase (GST). Recombinant VaGA2oxA1 (rVaGA2oxA1) and rVaGA2oxA2 showed 2β-hydroxylation activity by converting GA1, GA4, GA9, GA20, GA4-methyl ester, and 16,17-dihydro-GA4 to the corresponding 2… 
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Isolation and expression of GA 2-oxidase2 in tomato

  • J. XiaoJ. H. Zhang Z. Ye
  • Biology, Medicine
    DNA sequence : the journal of DNA sequencing and mapping
  • 2007
Semi-quantitative RT-PCR analysis showed a specific expression profile of SlGA2ox2 in different tissues, which mainly expressed in flowers and traces were detected in roots, stems, leaves and immature fruits.

Screening and characterization of an inhibitory chemical specific to Arabidopsis gibberellin 2-oxidases.

Gibberellin receptor and its role in gibberellin signaling in plants.

It has been concluded that GID1 is a soluble receptor mediating GA signaling in rice, which binds to SLR1, a repressor of GA signaling, in a GA-dependent manner in yeast cells.

Trails to the gibberellin receptor, GIBBERELLIN INSENSITIVE DWARF1

The history of the research on GA receptor from the early attempts to the identification of GID1 is described, suggesting that the active principle of the gibberellin-binding protein was a G ID1 homolog.

Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt

The findings suggest that EVA inhibits angiogenesis in VEGF-treated HUVECs via inhibition of phosphorylation of VEGFR2, ERK, and Akt, and overall, the antiangiogenic mechanism of EVA is suggested to be inhibited.

Antheridiogen determines sex in ferns via a spatiotemporally split gibberellin synthesis pathway

It is determined that ferns have evolved an antheridiogen-mediated communication system to produce males by modifying the gibberellin biosynthetic pathway, which is split between two individuals of different developmental stages in the colony.

Identification of a Peptide Mimic of Bioactive Gibberellins with Affinity to GA 2-Oxidase

The first example of peptide mimics of a small hydrophobic molecule, a phytohormone gibberellin, has been reported and the second peptide mimic has been identified from random peptide libraries by its affinity to a type of catalyzing enzyme of gibBerellins, which specifically recognizes bioactive gibb Berellins.

Interacción de Giberelinas y Auxinas en la fructificación del tomate.

El efecto de la aplicacion de giberelinas (GAs) y auxinas durante la fructificacion y desarrollo del fruto, ha sido investigado en tomate (Solanum lycopersicum L.) cv Micro-Tom. Los resultados…

Distribution and prediction of catalytic domains in 2-oxoglutarate dependent dioxygenases

  • S. Kundu
  • Biology, Computer Science
    BMC Research Notes
  • 2012
The proposed stratification is a novel attempt at classifying and predicting 2-oxoglutarate dependent function and will aid efforts by investigators to screen and characterize putative 2-OG dependent sequences by helping to compare data to a comprehensive list of HMM profiles of catalytic domains.

Similarities and Differences between the Characteristics of Gibberellin-Binding Protein and Gibberellin 2-Oxidases in Adzuki Bean (Vigna angularis) Seedlings

A major component that showed GA-binding activity was apparently different from any gibberellin 2-oxidase cloned from the seedlings, and it was not affected by the antibody treatment.

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