Giardia lamblia: a culture method for determining parasite viability.

  title={Giardia lamblia: a culture method for determining parasite viability.},
  author={David R Hill and Roderike Pohl and Richard D. Pearson},
  journal={The American journal of tropical medicine and hygiene},
  volume={35 6},
A simple, quantitative method has been developed to assess the viability of Giardia lamblia trophozoites after exposure to a potentially lethal agent. This method utilizes the ability of trophozoites which remain viable to replicate and multiply. In this study, the percent dead trophozoites after incubation in 20% fresh human serum was determined first by morphologic criteria and then by the ability of parasites to multiply in fresh medium over 48 hr. Using the number of parasites after 48 hr… CONTINUE READING

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