Giardia gene predicts a 183 kDa nucleotide-binding head-stalk protein.

  title={Giardia gene predicts a 183 kDa nucleotide-binding head-stalk protein.},
  author={J S Marshall and David V. Holberton},
  journal={Journal of cell science},
  volume={108 ( Pt 7)},
Previously described extended proteins from the cytoskeleton of Giardia lamblia (beta-giardin, median body protein) have been found to be segmented coiled coils with regular structural repeat patterns in their amino acid sequences. Screening a lambda ZAPII library derived from Giardia genomic DNA with an antibody directed against a 34 x 10(3) M(r) giardin isoform selected a gene encoding a much larger polypeptide chain (HPSR2), the sequence of which was determined by chromosome walking the open… CONTINUE READING
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