Giant unilamellar vesicle electroformation from lipid mixtures to native membranes under physiological conditions.

@article{Mlard2009GiantUV,
  title={Giant unilamellar vesicle electroformation from lipid mixtures to native membranes under physiological conditions.},
  author={Philippe M{\'e}l{\'e}ard and Luis A. Bagatolli and Tanja Pott},
  journal={Methods in enzymology},
  year={2009},
  volume={465},
  pages={161-76}
}
Giant unilamellar vesicles (GUVs) are well-known model systems, especially because they are easily observable using optical microscopy. In this chapter, we revisit in detail the versatile GUV electroformation protocol. We demonstrate how GUV electroformation can be adapted to various membrane systems including synthetic lipid mixtures, natural lipid extracts, and bilayers containing membrane proteins. Further, we show how to adjust this protocol to a given aqueous environment and prove that… CONTINUE READING