Giant reticulospinal synapse in lamprey: molecular links between active and periactive zones

@article{Brodin2006GiantRS,
  title={Giant reticulospinal synapse in lamprey: molecular links between active and periactive zones},
  author={L. Brodin and Oleg Shupliakov},
  journal={Cell and Tissue Research},
  year={2006},
  volume={326},
  pages={301-310}
}
Deciphering the function of synaptic release sites is central to understanding neuronal communication. Here, we review studies of the lamprey giant reticulospinal synapse, a model that can be used to dissect synaptic vesicle trafficking at single release sites. The presynaptic axon is large and contains active zones that are spatially separated from each other. During activity, synaptic vesicle membrane is shuttled between the active zone and the periactive zone at which endocytosis occurs… Expand
From the DEPARTMENT OF NEUROSCIENCE Karolinska Institutet, Stockholm, Sweden MOLECULAR MECHANISMS IN SYNAPTIC VESICLE RECYCYLING
Neurons communicate through specialized contacts called synapses, which consist of preand postsynaptic compartments separated by a narrow synaptic cleft. The stimulusdependent rapid influx of calciumExpand
The Presynaptic Active Zone
TLDR
This review discusses the molecular composition and function of the active zone of a presynaptic nerve terminal, which mediates short- and long-term plasticity in response to bursts of action potentials, thus critically contributing to the computational power of a synapse. Expand
STUDIES OF MEMBRANE-BINDING PROTEINS INVOLVED IN SYNAPTIC VESICLE RECYCLING
Signaling between neurons occurs at synapses. A presynaptic nerve terminal releases neurotransmitter, which diffuses over the synaptic cleft, and binds to postsynaptic receptors. Release occurs byExpand
Role of epsin 1 in synaptic vesicle endocytosis
TLDR
It is concluded that epsin is involved in clathrin-mediated synaptic vesicle endocytosis, and a model, based on in vitro studies, is suggested that ePSin coordinates curvature generation with coat assembly and further indicating that Epsin limits clathin coat assembly to the size of newly formed vesicles. Expand
Perturbation of Syndapin/PACSIN Impairs Synaptic Vesicle Recycling Evoked by Intense Stimulation
TLDR
The data show an involvement of syndapin in synaptic vesicle recycling evoked by intense stimulation, which is proposed to be required to stabilize the plasma membrane and/or facilitate bulk endocytosis at high release rates. Expand
Piccolo Directs Activity Dependent F-Actin Assembly from Presynaptic Active Zones via Daam1
TLDR
Daam1, a Formin that functions with Profilin to drive F-actin assembly, is identified as a novel Piccolo binding partner, and it is found that within cells Daam1 activation promotesPiccolo binding, an interaction that can spatially direct the polymerization of F-Actin. Expand
The synapsin cycle: A view from the synaptic endocytic zone
TLDR
It is demonstrated that synapsins migrate to the endocytic zone of central synapses during neurotransmitter release, which suggests that there are additional functions for these proteins in SV recycling. Expand
Synaptic Vesicle Endocytosis in Different Model Systems
TLDR
Comparing model systems based on well-characterized model systems in terms of their known modes and kinetics of synaptic vesicle endocytosis, as well as the underlying molecular machineries are compared to provide the future development of this field. Expand
A readily retrievable pool of synaptic vesicles
TLDR
This study monitored the temporal dynamics of a presorted and preassembled pool of synaptic vesicle proteins on the presynaptic membrane in rat hippocampal neurons using a new type of probe and demonstrated the preferential recruitment of a surface pool of neurotransmitter proteins upon stimulated endocytosis. Expand
Single calcium channel nanodomains drive presynaptic calcium entry at lamprey reticulospinal presynaptic terminals
TLDR
1:1 association of Ca2+ channels with primed vesicles is indicated, suggesting Ca2- entry via as few as one channel may trigger neurotransmitter release in simple active zone synapses. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 65 REFERENCES
Ultrastructural organization of lamprey reticulospinal synapses in three dimensions
TLDR
The giant reticulospinal synapse in lamprey provides a unique model to study synaptic vesicle traffic and the vast majority of active zones in all synapse types contained both chemical and electrotonic synaptic specializations. Expand
Colocalization of synapsin and actin during synaptic vesicle recycling
TLDR
The data suggest that in addition to its known function in clustering of vesicles in the reserve pool, synapsin migrates from the synaptic vesicle cluster and participates in the organization of the actin-rich cytomatrix in the endocytic zone during synaptic activity. Expand
The actin cytoskeleton and neurotransmitter release: an overview.
TLDR
There is no evidence that actin participates in fusion of synaptic vesicles, but actin might be a pivotal participant in calcium signaling inside presynaptic nerve terminals. Expand
Molecular organization of the presynaptic active zone
TLDR
Five protein families whose members are highly enriched at active zones (Munc13s, RIMs, ELKS proteins, Piccolo and Bassoon, and the liprins-α), have been characterized and are instrumental for the diverse functions performed by the presynaptic active zone. Expand
Amphiphysin is a Component of Clathrin Coats Formed During Synaptic Vesicle Recycling at the Lamprey Giant Synapse
TLDR
It is shown that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity, and is a component of presynaptic clathrin‐coated intermediates formed during synapticvesicle recycling. Expand
Location and function of vesicle clusters, active zones and Ca2+ channels in the lamprey presynaptic terminal
TLDR
At lamprey giant reticulospinal synapses, Ca2+ channels and release sites colocalize, creating a close spatial relationship between active zones and Ca2- entry sites, which is necessary for site‐specific, Ca1+‐dependent secretion. Expand
Chapter X A model glutamate synapse—the lamprey giant reticulospinal axon
TLDR
This chapter describes the lamprey giant reticulospinal synapse, which utilizes glutamate as a neurotransmitter and has been used as a model system to determine the subcellular localization and uptake of glutamate in synaptic regions. Expand
Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton
TLDR
Evidence is provided for a critical role of actin in recycling of synaptic vesicle recycling, which seems to involve functions both in endocytosis and in the transport of recycled vesicles to the synapticvesicle cluster. Expand
The cytoskeletal architecture of the presynaptic terminal and molecular structure of synapsin 1
TLDR
Data derived via three different approaches suggest that synapsin 1 could be a main element of short linkages between actin filaments and synaptic vesicles, and between microtubules and synaptic Vesicles in the nerve terminals. Expand
A pre-embedding immunogold approach for detection of synaptic endocytic proteins in situ
TLDR
The experiments show that the current pre-embedding immunogold method is a useful experimental tool to study the molecular mechanisms of synaptic vesicle recycling, and confirms the presence of dynamin on late stage coated pits and demonstrates that dynamin is recruited to the coat of endocytic intermediates from the very early stages of the clathrin coat formation. Expand
...
1
2
3
4
5
...