Giant Vesicles: Preparations and Applications

  title={Giant Vesicles: Preparations and Applications},
  author={Peter Walde and Katia Cosentino and Helen Engel and Pasquale Stano},
There is considerable interest in preparing cell‐sized giant unilamellar vesicles from natural or nonnatural amphiphiles because a giant vesicle membrane resembles the self‐closed lipid matrix of the plasma membrane of all biological cells. Currently, giant vesicles are applied to investigate certain aspects of biomembranes. Examples include lateral lipid heterogeneities, membrane budding and fission, activities of reconstituted membrane proteins, or membrane permeabilization caused by added… 

Generating Giant Membrane Vesicles from Live Cells with Preserved Cellular Properties

This study develops a facile strategy to derive giant membrane vesicles (GMVs) from mammalian cells in biofriendly medium with high yields and highlights biocompatible GMVs with biomimicking membrane surface properties and adaptability as an ideal platform for drug delivery strategies with potential clinical applications.

Giant liposome formation toward the synthesis of well-defined artificial cells.

An overview of giant liposome formation using microfluidic technologies is provided, including its effects on the monodispersion, encapsulation efficiency, size range, and asymmetry of membranes.

Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles

Using fusion of oppositely charged membranes to reconstitute a model membrane protein, it is found that empty vesicles and proteoliposomes show similar fusion behavior, which allows for a rapid estimation of protein incorporation using fluorescent lipids.

Facile generation of giant unilamellar vesicles using polyacrylamide gels

It is demonstrated that the gentle hydration of PAA gels results in predominantly unilamellar vesicles, which is in contrast to comparable methods analyzed in this work.

Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction

The quality of the giant vesicles encapsulating a cell-free protein expression system was comparable to that of the ones formed using a conventional method, in terms of the synthesis of both soluble and membrane proteins.

Gel-assisted formation of giant unilamellar vesicles.

Model membrane systems, membrane-active peptides and application possibilities

Modelling the membrane interactions and perturbation mechanism of two structural modified variants of the well-described CPP sC18, a cyclized and a dimeric branched version, with artificial model membranes suggest that the internalization of the peptide variants depends highly on the membrane composition of the target cell.

Giant unilamellar vesicles formed by hybrid films of agarose and lipids display altered mechanical properties.




Protein Incorporation in Giant Lipid Vesicles under Physiological Conditions

Two new applications of a specialized electroformation method are presented that can produce giant vesicles under physiologically relevant salt conditions by applying an electric field with a high frequency and show that the encapsulated proteins not only retain their function but also give shape to the GVs.

Characterization of giant vesicles formed by phase transfer processes

Vesicles are of great interest as drug delivery system or models for cell membranes. For many applications, it is necessary to produce vesicles which are unilamellar, monodisperse, easy to adjust in

Spontaneous Generation of Giant Liposomes from an Oil/Water Interface

It is observed that giant liposomes spontaneously formed when a horizontal liquid interface was made between an upper oil phase containing phospholipid and a lower water phase, a new spontaneous phenomenon in a biphasic (oil/water) environment.

Engineering asymmetric vesicles

This work describes the systematic engineering of unilamellar vesicles assembled with two independently prepared monolayers, which produces asymmetries as high as 95% and demonstrates the versatility of this method by investigating the stability of the asymmetry.

Giant unilamellar vesicles electroformed from native membranes and organic lipid mixtures under physiological conditions.

It is shown for first time that giant unilamellar vesicles can be prepared using a new protocol based on the electroformation method either from native membranes or organic lipid mixtures at physiological ionic strength.

Unilamellar vesicle formation and encapsulation by microfluidic jetting

The ability of microfluidic jetting to controllably encapsulate solutions inside of GUVs creates new opportunities for the study and use of compartmentalized biomolecular systems in science, industry, and medicine.

A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy.

The current understanding of lipid bilayers as obtained from studies on giant unilamellar vesicles is reviewed and some recent developments on curvature effects induced by polymers, domain formation in membranes and shape transitions induced by electric fields are summarized.

Artificial cells: Unique insights into exocytosis using liposomes and lipid nanotubes

The use of liposome–lipid nanotube networks are described to create an artificial cell model that undergoes the later stages of exocytosis and shows that membrane mechanics, without protein intervention, can drive expansion of the fusion pore to the final stage of excycletosis.