Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis.

  • Yasunori Sasakura
  • Published 2007 in
    Developmental dynamics : an official publication…


Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines, enhancer/gene traps, and insertional mutagenesis. Recently, transposon-mediated transformation using a Tc1/mariner transposable element Minos has been reported in two ascidians: Ciona intestinalis and C. savignyi. The transposon derived from an insect… (More)


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