Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector

@article{Tamura2000GermlineTO,
  title={Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector},
  author={Toshiki Tamura and Chantal Thibert and Corinne Royer and Toshio Kanda and Abraham Eappen and Mari Kamba and Natuo K{\^o}moto and Jean-Luc Thomas and Bernard Mauchamp and G{\'e}rard Chavancy and Paul D Shirk and Malcolm Fraser and Jean-Claude Prudhomme and Pierre Couble},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={81-84}
}
We have developed a system for stable germline transformation in the silkworm Bombyx mori L. using piggyBac, a transposon discovered in the lepidopteran Trichoplusia ni. The transformation constructs consist of the piggyBac inverted terminal repeats flanking a fusion of the B. mori cytoplasmic actin gene BmA3 promoter and the green fluorescent protein (GFP). A nonautonomous helper plasmid encodes the piggyBac transposase. The reporter gene construct was coinjected into preblastoderm eggs of two… CONTINUE READING
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