Germline stem cells and follicular renewal in the postnatal mammalian ovary

@article{Johnson2004GermlineSC,
  title={Germline stem cells and follicular renewal in the postnatal mammalian ovary},
  author={Joshua Johnson and Jacqueline Canning and T. Kaneko and J. Pru and J. Tilly},
  journal={Nature},
  year={2004},
  volume={428},
  pages={145-150}
}
A basic doctrine of reproductive biology is that most mammalian females lose the capacity for germ-cell renewal during fetal life, such that a fixed reserve of germ cells (oocytes) enclosed within follicles is endowed at birth. Here we show that juvenile and adult mouse ovaries possess mitotically active germ cells that, based on rates of oocyte degeneration (atresia) and clearance, are needed to continuously replenish the follicle pool. Consistent with this, treatment of prepubertal female… Expand

Figures and Topics from this paper

Paper Mentions

Interventional Clinical Trial
Two group of patients were selected. Group 1 represents 46 patients that received ovarian-biostimulation monthly for three months. Group 2 represents 32 patients that received… Expand
ConditionsOvarian Reserve
InterventionDietary Supplement, Procedure
Interventional Clinical Trial
Women delay maternity and, as a consequence, available oocyte number and their quality decrease (9-18% of all IVF patients). Different treatment protocols have been developped… Expand
ConditionsOvarian Reserve
InterventionProcedure
Female mice lack adult germ-line stem cells but sustain oogenesis using stable primordial follicles
TLDR
Adult female mice neither require nor contain active germ-line stem cells or produce new oocytes in vivo, and this method directly identifies active geriatric stem cells. Expand
Purification of germline stem cells from adult mammalian ovaries: a step closer towards control of the female biological clock?
TLDR
Results from transplantation studies demonstrated that proliferative germ cells resembling male spermatogonial stem cells generate oocytes in ovaries of chemotherapy-sterilized recipients that fertilize and produce viable offspring, and strongly support the existence of GSC in adult mouse ovaries. Expand
Oocyte formation by mitotically-active germ cells purified from ovaries of reproductive age women
TLDR
A fluorescence-activated cell sorting-based protocol that can be used with adult mouse ovaries and human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells is described and validated. Expand
Oocyte Generation in Adult Mammalian Ovaries by Putative Germ Cells in Bone Marrow and Peripheral Blood
TLDR
It is shown that adult mouse ovaries rapidly generate hundreds of oocytes, despite a small premeiotic germ cell pool, and bone marrow transplantation restores oocyte production in wild-type mice sterilized by chemotherapy, as well as in ataxia telangiectasia-mutated gene-deficient mice, which are otherwise incapable of making oocytes. Expand
From primordial germ cell to primordial follicle: mammalian female germ cell development
TLDR
This review focuses on the early stages of female germ cell development, which is poorly understood but molecules and mechanisms that regulate oocyte development are beginning to be identified. Expand
Production of offspring from a germline stem cell line derived from neonatal ovaries
TLDR
A neonatal mouse FGSC line is established, with normal karyotype and high telomerase activity, by immunomagnetic isolation and culture for more than 15 months, which contribute to basic research into oogenesis and stem cell self-renewal and open up new possibilities for use of FGSCs in biotechnology and medicine. Expand
Ovulated oocytes in adult mice derive from non-circulating germ cells
TLDR
These studies showed no evidence that bone marrow cells, or any other normally circulating cells, contribute to the formation of mature, ovulated oocytes, and cells that travelled to the ovary through the bloodstream exhibited properties characteristic of committed blood leukocytes. Expand
Quantification of healthy follicles in the neonatal and adult mouse ovary: evidence for maintenance of primordial follicle supply.
TLDR
The hypothesis of postnatal follicle renewal in postnatal and adult ovaries of C57BL/6 mice is supported and no evidence for ovarian germline stem cells is found. Expand
Oogenesis in adult mammals, including humans
TLDR
The data indicate that the pool of primary follicles in adult human ovaries does not represent a static but a dynamic population of differentiating and regressing structures, and the follicular renewal may cease at a certain age, and this may predetermine the onset of the natural menopause or premature ovarian failure. Expand
Aged mouse ovaries possess rare premeiotic germ cells that can generate oocytes following transplantation into a young host environment
TLDR
It is shown that aged mouse ovaries possess a rare population of premeiotic germ cells that retain the capacity to form oocytes if exposed to a young host environment. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 72 REFERENCES
Mouse ovarian germ cell cysts undergo programmed breakdown to form primordial follicles.
TLDR
It is shown that mouse cysts undergo programmed breakdown between 20.5-22.5 dpc, during which approximately 33% of the oocytes survive to form primordial follicles, which suggests that perinatal germ cell loss is a developmentally regulated process that is distinct from the follicular atresia that occurs during adult life. Expand
Ablation of bcl-2 gene expression decreases the numbers of oocytes and primordial follicles established in the post-natal female mouse gonad.
TLDR
Histological analyses revealed that ovaries collected from bcl-2 -/- mice possessed numerous aberrantly formed primordial follicle-like structures containing a single layer of granulosa cells without an oocyte, suggesting that expression of the bCl-2 death repressor gene is critical for endowment of a normal complement of germ cells and primordial feathers in the mammalian ovary. Expand
Meiosis and differentiation of mouse germ cells.
  • A. Mclaren
  • Biology, Medicine
  • Symposia of the Society for Experimental Biology
  • 1984
The female pathway of germ cell development is characterized by early entry into meiotic prophase, before birth in the mouse. This pathway is followed by all germ cells in the ovary and in theExpand
The tao of stem cells in the germline.
  • H. Lin
  • Biology, Medicine
  • Annual review of genetics
  • 1997
TLDR
The establishment and self-renewing division of GSCs are controlled by extracellular signals such as hormones from the hypothalamic-pituitary axis and local interactions between G SCs and their neighboring cells. Expand
Prolongation of ovarian lifespan into advanced chronological age by Bax-deficiency
TLDR
It is shown that young adult female Bax–/– mice possess threefold more primordial follicles in their ovarian reserve than their wild–type sisters, and this surfeit of follicles is maintained in advanced chronological age, such that 20–22–month–old female BAX–/- mice possess hundreds of follicle at all developmental stages and exhibit ovarian steroid–driven uterine hypertrophy. Expand
Restoration of Fertility by Germ Cell Transplantation Requires Effective Recipient Preparation1
TLDR
This approach was effective because the cytoablative treatment reduced (but did not abolish) endogenous spermatogenesis, creating space for colonization by donor stem cells, and residual endogenous germ cells contributed to a healthy testicular environment that supported robust donor and recipient sperMatogenesis. Expand
Spermatogenesis following male germ-cell transplantation.
  • R. Brinster, J. Zimmermann
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1994
TLDR
It is reported here that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules and may prove useful as a tool for biomedical science and biotechnology. Expand
Female Germ Cell Aneuploidy and Embryo Death in Mice Lacking the Meiosis-Specific Protein SCP3
TLDR
It is found that SCP3 is required for chiasmata formation and for the structural integrity of meiotic chromosomes, suggesting that altered chromosomal structure triggers nondisjunction and is thus linked to inherited aneuploidy in female germ cells and provides a model system for studying age-dependent degeneration in oocytes. Expand
Imminent oocyte exhaustion and reduced follicular recruitment mark the transition to acyclicity in aging C57BL/6J mice.
TLDR
Quantitative cytological analyses of aging C57BL/6J mouse ovaries revealed that the populations of primordial and growing follicles were nearly exhausted by 13-14 months, the average age of ovulatory failure, suggesting that follicular depletion is a factor which contributes to the loss of ovarian cyclicity during aging. Expand
Requirement for phosphatidylinositol-3'-kinase in cytokine-mediated germ cell survival during fetal oogenesis in the mouse.
TLDR
The data indicate that the combined actions of SCF, LIF, and IGF-I are required for maximal inhibition of apoptosis in germ cells of fetal mouse ovaries, and that the PI3K signaling pathway is an essential component of cytokine-mediated female germ cell survival. Expand
...
1
2
3
4
5
...