Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

@article{Lois2002GermlineTA,
  title={Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors},
  author={Carlos Lois and Elizabeth J. Hong and Shirley Pease and Eric J. Brown and David Baltimore},
  journal={Science},
  year={2002},
  volume={295},
  pages={868 - 872}
}
Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte–specific promoters… 

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...

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