Germline Stem Cells Anchored by Adherens Junctions in the Drosophila Ovary Niches

@article{Song2002GermlineSC,
  title={Germline Stem Cells Anchored by Adherens Junctions in the Drosophila Ovary Niches},
  author={Xiaoqing Song and Chun-hong Zhu and C. Doan and Ting Xie},
  journal={Science},
  year={2002},
  volume={296},
  pages={1855 - 1857}
}
How stem cells are recruited to and maintained in their niches is crucial to understanding their regulation and use in regenerative medicine. Here, we demonstrate that DE-cadherin–mediated cell adhesion is required for anchoring germline stem cells (GSCs) in their niches in the Drosophila ovary. Two major components of this adhesion process, DE-cadherin and Armadillo/β-catenin, accumulate at high levels in the junctions between GSCs and cap cells, one of the niche components. Removal of these… 

DE-cadherin-mediated cell adhesion is essential for maintaining somatic stem cells in the Drosophila ovary

  • Xiaoqing SongT. Xie
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
It is shown that cadherin-mediated cell adhesion is required for anchoring somatic stem cells (SSCs) to their niches in the Drosophila ovary, providing further support that SSCs are located in a niche formed by their neighboring cells.

The receptor tyrosine phosphatase Lar regulates adhesion between Drosophila male germline stem cells and the niche

TLDR
It is shown that the transmembrane receptor tyrosine phosphatase Leukocyte-antigen-related-like (Lar), which is best known for its function in axonal migration and synapse morphogenesis in the nervous system, helps maintain G SCs at the hub by promoting E-cadherin-based adhesion between hub cells and GSCs.

Stem cells, niches and cadherins: a view from Drosophila

TLDR
Recent work shows that DE-cadherin-mediated adhesion is used for the initial recruitment and posterior anchoring of the germline-derived stem cells in their niche.

Integrins control the positioning and proliferation of follicle stem cells in the Drosophila ovary

TLDR
This study shows that follicle stem cells in the Drosophila melanogaster ovary rely on cues that are distinct from those of other ovarian stem cells to establish and maintain their unique niche, and supports a model in which FSCs contribute to the formation and maintenance of their own niche by producing the integrin ligand, laminin A (LanA).

Anchoring stem cells in the niche by cell adhesion molecules

  • R. Xi
  • Biology
    Cell adhesion & migration
  • 2009
TLDR
Understanding stem cell anchorage and release in coupling with environmental changes should provide further insights into homeostasis control in tissues that harbor stem cells.

Rab11 maintains connections between germline stem cells and niche cells in the Drosophila ovary

TLDR
It is proposed that this association polarizes the trafficking by Rab11 of E-cadherin and other cargoes toward the anterior cortex of the GSC, thus simultaneously fortifying GSC-niche junctions, fusome localization and asymmetric cell division.

Interactions between stem cells and their niche in the Drosophila ovary.

TLDR
The findings of the studies on interactions between Drosophila ovarian stem cells and their niches during normal development and aging and on stem cell competition for niche occupancy are highlighted.

Local BMP receptor activation at adherens junctions in the Drosophila germline stem cell niche.

TLDR
A novel fluorescence-based reporter is described that directly visualizes bone morphogenetic protein (BMP) receptor activation and shows that in the Drosophila testis a BMP niche signal is transmitted preferentially at adherens junctions between hub and germline stem cells, resembling the proposed synapse organization.

E-Cadherin Is Required for Centrosome and Spindle Orientation in Drosophila Male Germline Stem Cells

TLDR
It is proposed that E-cadherin orchestrates multiple aspects of stem cell behavior, including polarization of stem cells toward the stem cell-niche interface and adhesion ofstem cells to the niche supporting cells.
...

References

SHOWING 1-10 OF 22 REFERENCES

A niche maintaining germ line stem cells in the Drosophila ovary.

TLDR
It is shown that anterior ovariolar somatic cells comprising three cell types act as a germ line stem cell niche, and the ability to repopulate the niche increases the functional lifetime of o varioles in vivo.

hedgehog is required for the proliferation and specification of ovarian somatic cells prior to egg chamber formation in Drosophila.

TLDR
The hedgehog (hh) gene plays a role in regulating cell proliferation and specifying cell identity in diverse systems and is expressed at the extreme apical end of Drosophila ovarioles in terminal filament cells and a new group of associated somatic cells.

Stem cells find their niche

TLDR
Technical advances now make it possible to characterize small zones that maintain and control stem cell activity in several organs, including gonads, skin and gut, and promise to advance efforts to use stem cells therapeutically.

Drosophila oocyte localization is mediated by differential cadherin-based adhesion

TLDR
It is proposed that in wild-type follicles the oocyte competes successfully with its sister germline cells for contact to the posterior follicle cells, a sorting process driven by different concentrations of DE-cadherin, the first in vivo example of a cell-sorting process that depends on differential adhesion mediated by a cadherin.

The self-renewing mechanism of stem cells in the germline.

  • H. Lin
  • Biology
    Current opinion in cell biology
  • 1998

piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells.

TLDR
It is shown that piwi encodes a novel nucleoplasmic protein present in both somatic and germline cells, with the highly conserved C-terminal region essential for its function.

A role for the Drosophila segment polarity gene armadillo in cell adhesion and cytoskeletal integrity during oogenesis.

TLDR
Examination of the distribution of Armadillo in the Drosophila ovary demonstrated that this localization often parallels the location of cell-cell adhesive junctions, and Germ-line armadillo mutations appear to disrupt processes requiring cell adhesion and integrity of the actin cytoskeleton, consistent with a role for Armadillos in cell- cell adhesive junitions.

The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation.

TLDR
The results imply that Drosophila fusomes are required for ovarian cyst formation and suggest that membrane skeletal proteins regulate cystocyte divisions.