Germ-line transmission and expression of a human-derived yeast artificial chromosome

@article{Jakobovits1993GermlineTA,
  title={Germ-line transmission and expression of a human-derived yeast artificial chromosome},
  author={Aya Jakobovits and Amy L. Moore and Larry L. Green and German Vergara and Catherine E. Maynard-Currie and Harry A. Austin and Sue Klapholz},
  journal={Nature},
  year={1993},
  volume={362},
  pages={255-258}
}
INTRODUCTION of DNA fragments, hundreds of kilobases in size, into mouse embryonic stem (ES) cells would greatly advance the ability to manipulate the mouse genome. Mice generated from such modified cells would permit investigation of the function and expression of very large or crudely mapped genes. Large DNA molecules cloned into yeast artificial chromosomes (YACs) are stable and genetically manipulable within yeast1, suggesting yeast-cell fusion as an ideal method for transferring large DNA… CONTINUE READING