Germ cell deficiency causes testis cord differentiation in reconstituted mouse fetal ovaries.

@article{Hashimoto1990GermCD,
  title={Germ cell deficiency causes testis cord differentiation in reconstituted mouse fetal ovaries.},
  author={Naoko Hashimoto and R Kubokawa and Kosuke Yamazaki and Masahiro Noguchi and Yukio Kato},
  journal={The Journal of experimental zoology},
  year={1990},
  volume={253 1},
  pages={61-70}
}
Sex-reversal in fetal ovaries was studied by using a dissociation-reconstitution technique. Gonads of 12.5 gestation-day male and female mouse fetuses were dissociated into single cells. To eliminate germ cells, the dissociated cells were cultured for 14 h, and then somatic cells attached to culture dishes were harvested and aggregated by gyratory culture for 24 h. The aggregates were then transplanted into ovarian bursa in ovary-ectomized nude mice. The recovered explants were examined… CONTINUE READING