Germ-Line Recombination Activity of the Widely Used hGFAP-Cre and Nestin-Cre Transgenes

@inproceedings{Zhang2013GermLineRA,
  title={Germ-Line Recombination Activity of the Widely Used hGFAP-Cre and Nestin-Cre Transgenes},
  author={Jiong Yi Zhang and Pavel Dublin and Stephanie Griemsmann and Alexandra Klein and Ralph Brehm and Peter Bedner and Bernd K Fleischmann and Christian Steinh{\"a}user and Martin Theis},
  booktitle={PloS one},
  year={2013}
}
Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Immune quiescence of the brain is set by astroglial connexin 43.

The Journal of neuroscience : the official journal of the Society for Neuroscience • 2015
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-10 of 42 references

hGFAPcre transgenic mice for manipulation of glial and neuronal function in vivo

L Zhuo, M Theis, I varez-Maya, M Brenner, K Willecke
Genesis • 2001
View 5 Excerpts
Highly Influenced

Astroglial networks scale synaptic activity and plasticity.

Proceedings of the National Academy of Sciences of the United States of America • 2011
View 2 Excerpts

Role of

D Gosejacob, P Dublin, P Bedner, K Hüttmann, J Zhang
ASTROGLIAL • 2011

Similar Papers

Loading similar papers…