Germ-Line Recombination Activity of the Widely Used hGFAP-Cre and Nestin-Cre Transgenes

  title={Germ-Line Recombination Activity of the Widely Used hGFAP-Cre and Nestin-Cre Transgenes},
  author={Jiong Yi Zhang and Pavel Dublin and Stephanie Griemsmann and Alexandra Klein and Ralph Brehm and Peter Bedner and Bernd K Fleischmann and Christian Steinh{\"a}user and Martin Theis},
  booktitle={PloS one},
Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ… CONTINUE READING

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