Geometry–Force Control of Stem Cell Fate

@article{Worley2013GeometryForceCO,
  title={Geometry–Force Control of Stem Cell Fate},
  author={Kathryn E Worley and Anthony Certo and Leo Q. Wan},
  journal={BioNanoScience},
  year={2013},
  volume={3},
  pages={43-51}
}
Tissue engineering requires tight control of stem cell function. Among many physical signals such as stretch and perfusion, geometrical cues have received much attention and have widely been recognized as an important factor in scaffold design. Here we review a variety of approaches that control stem cell fate at different levels of strictness, including micro-contact printing, microwells, direct cell printing, grooves, aligned micro-/nano-fibers, nanotubes, nanodots, hydrogel shape, and porous… 

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References

SHOWING 1-10 OF 95 REFERENCES

Geometric control of stem cell differentiation rate on surfaces.

It is found that the pattern geometry and therefore the cell population, rather than the cell adhesive area, influence the rate of adipogenic differentiation from hMSC's.

Stem cell fate dictated solely by altered nanotube dimension

Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media.

Geometric control of human stem cell morphology and differentiation.

The role of substrate geometry on stem cell differentiation is confirmed, through associated physical forces, and provides a simple and controllable system for studying biophysical regulation of cell function.

BIOMIMETIC GRADIENT HYDROGELS FOR TISSUE ENGINEERING.

This review draws on specific biological examples to motivate hydrogel gradients as tools for studying cell-material interactions and summarizes the current and future trends in gradient hydrogels and cell- material interactions in context with the long-term goals of tissue engineering.

Cellular Mechanical Stress Gradient Regulates Cell Proliferation and Differentiation Patterns

This study was designed to determine the relationship between mechanical stress gradient and cell proliferation and differentiation patterns using theoretical modeling, cell traction force microscopy, and immuno-histochemistry.

Scaffolds for tissue engineering and 3D cell culture.

This chapter reviews the principal polymeric materials that are used for the fabrication of scaffolds and the scaffold fabrication processes, with examples of properties and selected applications.

The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder.

The use of nanoscale disorder is demonstrated to stimulate human mesenchymal stem cells (MSCs) to produce bone mineral in vitro, in the absence of osteogenic supplements, which has implications for cell therapies.
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