Geometric isomers of covalently labeled mitochondrial F1-adenosinetriphosphatase with different properties.


Two geometric isomers of covalently labeled F1-adenosinetriphosphatase (F1-ATPase) have been prepared by reaction with 7-chloro-4-nitro-2,1,3-benzoxadiazole (NBD-Cl): a directly labeled product denoted by O-beta'-NBD-F1 and an indirectly prepared product denoted by 0-beta'-NBD-F1. The normal isomer O-beta'-NBD-F1 is highly inhibited, and its label can be… (More)