• DOI:10.1099/ijs.0.039321-0
• Corpus ID: 20635255

Geobacter luticola sp. nov., an Fe(III)-reducing bacterium isolated from lotus field mud.

@article{Viulu2013GeobacterLS,
  title={Geobacter luticola sp. nov., an Fe(III)-reducing bacterium isolated from lotus field mud.},
  author={Samson Viulu and Kohei Nakamura and Yuri Okada and Sakiko Saitou and Kazuhiro Takamizawa},
  journal={International journal of systematic and evolutionary microbiology},
  year={2013},
  volume={63 Pt 2},
  pages={
          442-8
        }
}

• Samson Viulu, K. Nakamura, +2 authors K. Takamizawa
• Published 1 February 2013
• Biology
• International journal of systematic and evolutionary microbiology

A novel species of Fe(III)-reducing bacterium, designated strain OSK6(T), belonging to the genus Geobacter, was isolated from lotus field mud in Japan. Strain OSK6(T) was isolated using a solid medium containing acetate, Fe(III)-nitrilotriacetate (NTA) and gellan gum. The isolate is a strictly anaerobic, gram-negative, motile, straight rod-shaped bacterium, 0.6-1.9 µm long and 0.2-0.4 µm wide. The growth of the isolate occurred at 20-40 °C with optima of 30-37 °C and pH 6.5-7.5 in the presence of up to 0.5 g NaCl l(-1). The G+C content of the genomic DNA was determined by HPLC to be 59.7 mol%. The major respiratory quinone was MK-8. The major fatty acids were 16 : 1ω7c and 16 : 0. Strain OSK6(T) was able to grow with Fe(III)-NTA, ferric citrate, amorphous iron (III) hydroxide and nitrate, but not with fumarate, malate or sulfate as electron acceptors. Among examined substrates grown with Fe(III)-NTA, the isolate grew on acetate, lactate, pyruvate and succinate. Analysis of the near full-length 16S rRNA gene sequence revealed that strain OSK6(T) is closely related to Geobacter daltonii and Geobacter toluenoxydans with 95.6 % similarity to the type strains of these species. On the basis of phylogenetic analysis and physiological tests, strain OSK6(T) is described as a representative of a novel species, Geobacter luticola sp. nov.; the type strain is OSK6(T) ( = DSM 24905(T) = JCM 17780(T)).