Gentamicin-Containing Peptone-Yeast Extract Medium for Cocultivation of Hartmannella vermiformis ATCC 50256 and Virulent Strains of Legionella pneumophila.

@article{Wadowsky1995GentamicinContainingPE,
  title={Gentamicin-Containing Peptone-Yeast Extract Medium for Cocultivation of Hartmannella vermiformis ATCC 50256 and Virulent Strains of Legionella pneumophila.},
  author={Robert M. Wadowsky and Ling-yu Wang and Stella Laus and John N. Dowling and John M. Kuchta and Stanley J. States and Robert B. Yee},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 12},
  pages={
          4464-7
        }
}
We evaluated the use of peptone-yeast extract (PY) medium, different strains of Hartmannella vermiformis, and gentamicin in a coculture system to improve the discrimination of virulent and avirulent strains of Legionella pneumophila. H. vermiformis ATCC 50256 was unique among four strains of H. vermiformis, in that it multiplied equally well in Medium 1034 and PY medium (Medium 1034 without fetal calf serum, folic acid, hemin, and yeast nucleic acid and with a 50% reduction of peptone). However… CONTINUE READING

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