Genotyping of the platelet‐specific alloantigen HPA‐5 (Bra/Brb) using polymerase chain reaction with sequence‐specific primers (PCR‐SSP)

  title={Genotyping of the platelet‐specific alloantigen HPA‐5 (Bra/Brb) using polymerase chain reaction with sequence‐specific primers (PCR‐SSP)},
  author={Ming Hou and L Rosengren-Kogan and B Forsberg and Anders Elmgren and Lennart Rydberg and Jack Kutti and Hans Wadenvik},
  journal={European Journal of Haematology},
Abstract:  A DNA‐based one‐stage technique, polymerase chain reaction with sequence‐specific primers (PCR‐SSP) was developed for genotyping of the platelet specific alloantigen HPA‐5 (Bra/Brb). Sequence‐specific primers, matching the wild type and the point mutation responsible for the HPA‐5 (Bra/Brb) phenotype, were constructed. Conjointly a fragment of the gene coding for glycoprotein (GP) IIIa was amplified as an internal control of the enzyme reaction. Using these HPA‐5 (Bra/Brb) sequence… 
5 Citations

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Flow cytometry is a valuable tool for large‐scale detection of PlA1 (HPA‐ 1a), however, flow cytometry based on only one antiserum cannot distinguish between homozygous and heterozygous carriers of Pl a 1a.

HPA‐1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP): a rapid and simple technique

A DNA‐based method was developed to genotype donors for the human platelet antigens HPA‐la and ‐1b and the results were concordant with serological phenotyping and independent DNA analysis.

Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes

SUMMARY. Prenatal typing for the human platelet antigens‐1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA‐1 incompatibility in which

Localization of the Br Polymorphism on a 144 bp Exon of the GPIa Gene and Its Application in Platelet DNA Typing

Summary Alloimmunization against the human platelet alloantigen system Br (HPA-5) is the second most common cause of neonatal alloimmune thrombocytopenia (NAIT) in Caucasian populations. We have

Rapid genotyping of the five major platelet alloantigens by reverse dot-blot hybridization.

The reverse dot-blot (RDB) technique was used to successfully genotype women and family members with neonatal alloimmune thrombocytopenia and with posttransfusion purpura and to prenatally genotype the amniocytes from a fetus at risk for thromBocy topenia.

Human platelet antigen‐1 (Zw) typing using PCR‐RFLP

The PCR‐RFLP typing method could be clinically useful in a number of immunologically mediated platelet disorders, characterized by severe thrombocytopenia and in early prenatal diagnosis of neonatal alloimmune thromBocy Topenia.

Determination of human platelet antigen frequencies in the Dutch population by immunophenotyping and DNA (allele-specific restriction enzyme) analysis.

The PCR-ASRA described in this report is a practical and reliable technique for the determination of alleles that code for platelet antigen allotypes, at least in the Dutch population.


A patient who developed delayed cutaneous reactions to two different preparations of UH (including one preservative-free), a preservative -free LMWH and a synthetic heparinoid, pentosan sulphonic ester (Fibrocid R), has been described.

The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).

The identification of the nucleotide substitution that defines the Bra/Brb alloantigen system will now permit both pre- and postnatal diagnosis for Br phenotype, and it is confirmed that this polymorphism segregates with Br phenotype.