Genotyping of the platelet‐specific alloantigen HPA‐5 (Bra/Brb) using polymerase chain reaction with sequence‐specific primers (PCR‐SSP)

@article{Hou1996GenotypingOT,
  title={Genotyping of the platelet‐specific alloantigen HPA‐5 (Bra/Brb) using polymerase chain reaction with sequence‐specific primers (PCR‐SSP)},
  author={Ming Hou and L Rosengren-Kogan and B Forsberg and Anders Elmgren and Lennart Rydberg and Jack Kutti and Hans Wadenvik},
  journal={European Journal of Haematology},
  year={1996},
  volume={57}
}
Abstract:  A DNA‐based one‐stage technique, polymerase chain reaction with sequence‐specific primers (PCR‐SSP) was developed for genotyping of the platelet specific alloantigen HPA‐5 (Bra/Brb). Sequence‐specific primers, matching the wild type and the point mutation responsible for the HPA‐5 (Bra/Brb) phenotype, were constructed. Conjointly a fragment of the gene coding for glycoprotein (GP) IIIa was amplified as an internal control of the enzyme reaction. Using these HPA‐5 (Bra/Brb) sequence… 
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5 Citations

5 Citations

Genotyping for platelet‐specific antigens: techniques for the detection of single nucleotide polymorphisms

The genetic basis of the HPA alloantigens, the most commonly used genome typing techniques and their pitfalls, and future developments are discussed in this review.

Gene frequencies of the HPA‐3 and HPA‐5 platelet antigen alleles among the Amerindians

The determination of gene frequencies of the HPA systems in different populations allows inference of gene flows and genetic constitution of populations and the estimation of the risk of platelet‐specificalloimmunization.

Minor histocompatibility antigen HA-1 and HPA-5 polymorphisms in HLA-identical related bone marrow transplantation.

The number of platelet glycoprotein Ia molecules is associated with the genetically linked 807 C/T and HPA‐5 polymorphisms

This data indicates that the neutral 807 C/T (Phe224) polymorphism of the glycoprotein (GP)Ia gene has been recently associated with the number of GPIa molecules on the platelet surface and may be involved in alloimmune thrombocytopenias.

HPA‐5b (Bra) neonatal alloimmune thrombocytopenia in Quebec: incidence and clinical outcome in 31 cases

The aims of this study were to calculate the observed and expected incidence of NAITP in Quebec, Canada, and to evaluate the clinical outcome of infants with anti‐HPA‐5bNAITP.

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