Genotyping of single Cryptosporidium oocysts in sewage by semi-nested PCR and direct sequencing.

@article{Hashimoto2006GenotypingOS,
  title={Genotyping of single Cryptosporidium oocysts in sewage by semi-nested PCR and direct sequencing.},
  author={Atsushi Hashimoto and Hitomi Sugimoto and Shigemitsu Morita and Tsuyoshi Hirata},
  journal={Water research},
  year={2006},
  volume={40 13},
  pages={2527-32}
}
This study describes an approach for genotyping individual Cryptosporidium oocysts obtained from sewage. We isolated single immunofluorescent assay (IFA)-stained Cryptosporidium oocysts from sewage concentrate using glass capillary pipettes and inverted epifluorescence microscopy. Each isolated Cryptosporidium oocyst was analyzed by semi-nested PCR for the 18S rRNA gene and direct sequencing of the PCR products. A total of 74 of 107 oocysts isolated from sewage were genotyped successfully. Of… CONTINUE READING

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