Genotyping of Mycoplasma pneumoniae clinical isolates reveals eight P1 subtypes within two genomic groups.

@article{DorigoZetsma2000GenotypingOM,
  title={Genotyping of Mycoplasma pneumoniae clinical isolates reveals eight P1 subtypes within two genomic groups.},
  author={J. Wendelien Dorigo-Zetsma and Jeanette Dankert and Sebastian A J Zaat},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 3},
  pages={965-70}
}
Three methods for genotyping of Mycoplasma pneumoniae clinical isolates were applied to 2 reference strains and 21 clinical isolates. By a modified restriction fragment length polymorphism (RFLP) analysis of PCR products of the M. pneumoniae cytadhesin P1 gene, 5 subtypes were discriminated among 13 P1 type 1 strains and 3 subtypes were discriminated among 8 P1 type 2 strains. Sequence analysis of the 16S-23S rRNA gene spacer region and part of the 23S rRNA gene revealed one nucleotide… CONTINUE READING