Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.

@article{Intemann2009GenotypingOI,
  title={Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.},
  author={Christopher D. Intemann and Thorsten Thye and Juergen Sievertsen and Ellis Owusu-Dabo and R Horstmann and Christian Meyer},
  journal={BioTechniques},
  year={2009},
  volume={46 1},
  pages={
          58-60
        }
}
Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West… CONTINUE READING