Genotyping mutations in BmAChE3: A survey of organophosphate-resistant and -susceptible strains of Rhipicephalus (Boophilus) microplus.

@article{Temeyer2009GenotypingMI,
  title={Genotyping mutations in BmAChE3: A survey of organophosphate-resistant and -susceptible strains of Rhipicephalus (Boophilus) microplus.},
  author={Kevin Bruce Temeyer and Pia Untalan Olafson and Robert J Miller},
  journal={Journal of medical entomology},
  year={2009},
  volume={46 6},
  pages={1355-60}
}
Mutations I48L, I54V, R86Q, V137I, I492M, and T548A were identified previously in BmAChE3, a gene encoding acetylcholinesterase, from the organophosphate (OP) acaricide-resistant San Rommn strain of Rhipicephalus (Boophilus) microplus. Recombinant BmAChE3 acetylcholinesterase containing the R86Q mutation was shown to exhibit nearly 20-fold reduction in the rate of phosphorylation by paraoxon relative to the wild-type sequence. In addition, the R86Q mutation was present in resistant laboratory… CONTINUE READING