Genotyping Cryptococcus neoformans var. neoformans with specific primers designed from PCR-fingerprinting bands sequenced using a modified PCR-based strategy.

@article{Cogliati2000GenotypingCN,
  title={Genotyping Cryptococcus neoformans var. neoformans with specific primers designed from PCR-fingerprinting bands sequenced using a modified PCR-based strategy.},
  author={Massimo Cogliati and M Allaria and Anna Maria Tortorano and Maria Anna Viviani},
  journal={Medical mycology},
  year={2000},
  volume={38 2},
  pages={97-103}
}
Cryptococcus neoformans var. neoformans can be subdivided into six genotypes (VN1-VN6) based on different combinations of four major bands (420, 475, 540 and 800 bp) obtained by polymerase chain reaction (PCR) fingerprinting using the (GACA)4 primer. The aim of this study was to identify specific primers able to amplify these bands. A modified PCR-based… CONTINUE READING