Genotypic response of cowpea Vigna unguiculata (L.) to in vitro regeneration from cotyledon explants

  title={Genotypic response of cowpea Vigna unguiculata (L.) to in vitro regeneration from cotyledon explants},
  author={Mohanjeet S. Brar and Jameel Muhammad Al-Khayri and Teddy E. Morelock and Edwin J. Anderson},
  journal={In Vitro Cellular & Developmental Biology - Plant},
A plant regeneration system applicable to 17 cowpea genotypes was developed. Cotyledons were initiated on 1/3 MS medium containing 15 to 35 mg N6-benzyladenine (BA) per 1 (66.6 to 155.3 µM) for 5 to 15 d. For shoot regeneration, the explants were transferred to a medium containing 1 mg BA per 1 (4.4 µM). Within 1 wk, shoot formation was visible at the proximal end of the cotyledons. Regeneration percentages (1% to 11%) and the numbers of shoots (4 to 12 per explant) were significantly… CONTINUE READING
Highly Cited
This paper has 64 citations. REVIEW CITATIONS


Publications citing this paper.
Showing 1-10 of 20 extracted citations

64 Citations

Citations per Year
Semantic Scholar estimates that this publication has 64 citations based on the available data.

See our FAQ for additional information.


Publications referenced by this paper.
Showing 1-10 of 10 references

Tolerance to blackeye cowpea mosaic potyvirus not correlated with decreased virus accumulation or protection from cowpea stunt disease

E. J. Anderson, A. S. Kline, E. Morelock.T., R. W. McNew
Plant Dis . • 1996

Particle bombardment.

Methods in cell biology • 1995

Somatic embryogenesis in cell suspension culture of cowpea [ Vigna unguiculata ( L . ) Walp . ]

S. Kulothungan, A. Ganapathi, A. Shajahan, K. Kathiravan
Israel J . Plant Sci . • 1995

In vitro culture of higher plants

M. Pierik.R.L.

Genotype - dependent response of spinach cuhivars to in vitro callus induction and plant regeneration

E H. Huang, T. E. Morelock, T. A. Busharar, E. E. Gbur
Plant Sci . • 1991

revised medium for rapid growth and bioassays with tobacco tissue cultures

T. Murashige, F. A. Skoog
Physiol . Plant . • 1962