Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S. pneumoniae.

@article{Suzuki2006GenotypicIO,
  title={Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S. pneumoniae.},
  author={Nao Suzuki and Mayumi Yuyama and Sinsaku Maeda and Haruhiko Ogawa and Kazuyuki Mashiko and Yusuke Kiyoura},
  journal={Journal of medical microbiology},
  year={2006},
  volume={55 Pt 6},
  pages={709-14}
}
It was previously reported that two oligonucleotide primer sets (spn9802 and spn9828) for discriminating Streptococcus pneumoniae from pneumococcus-like oral streptococcal isolates using PCR had been developed. In this study, PCR amplification of the lytA, ply, spn9802 and spn9828 genes was used to identify presumptive S. pneumoniae. Two genetic groups were identified by analysing sputum samples from 28 patients with community-acquired pneumonia: the lytA-positive, ply-positive, spn9802… CONTINUE READING

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